Coding

Part:BBa_K1039022:Design

Designed by: Jama Hagi-Yusuf   Group: iGEM13_Waterloo   (2013-09-13)


PhiC31 integrase and PhiC31 RDF under lock and key control with lacI Expression Cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
    Illegal XhoI site found at 1519
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4498
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3619
    Illegal SapI site found at 3133
    Illegal SapI site found at 4164
    Illegal SapI site found at 4386
    Illegal SapI site found at 4512
    Illegal SapI.rc site found at 2824
    Illegal SapI.rc site found at 2882
    Illegal SapI.rc site found at 2987


Design Notes

For PhiC31 RDF, a base was added after the stop codon to prevent creation of an SpeI site due to addition of suffix. For PhiC31 integrase, there was a silent mutation on base 52; changed from T to C.


Source

The parts to this sequenced were either synthesized by BioBasic Inc, or from the iGEM distribution kit.

References